Figure 7

Perturbed relative responses to different central regulators in the Gpr54^−/−Tg rescued mouse.

Relative LH responses to different central regulators of the gonadotropic axis in adult WT, Gpr54^−/−Tg rescued and Gpr54^−/− null male mice are shown. The animals were subjected to central (icv) injection of effective doses of kisspeptin-10 (Kp-10), NMDA (agonist of ionotropic glutamate receptors), PHP (antagonist of GABA-A receptors), or agonists of the tachykinin receptors, NK1R, NK2R and NK3R. Hormonal levels were assayed 15-min after icv administration of the compounds. Animals injected with vehicle (Veh) served as controls to set basal hormonal levels. Considering the notable differences in basal LH levels among the three genotypes, hormonal responses were normalized by the corresponding basal levels (set at 0 line), to express relative increments of LH secretion (ΔLH). a P < 0.01 vs. corresponding stimulated values in WT Gpr54+/+ mice (ANOVA followed by Student-Newman-Keuls multiple range test). ND: No increment detected.