Table 1 Mutation of the “headlock” motif leads to an increased dissociation. The table summarizes affinities (KD), association (kon) and dissociation constants (koff) determined for BC2-Nb, BC2-NbR106E and BC2-NbR106S. Corresponding sensograms are shown in Supplementary Figure 3.

From: Peptides in headlock – a novel high-affinity and versatile peptide-binding nanobody for proteomics and microscopy

Nbs

Rmax [RU]

KD [nM]

kon [M−1s−1]

koff [s−1]

Chi2

BC2-Nb

170 ± 0.34

1.4 ± 0.06

4.6 ± 0.04 × 105

6.4 ± 0.27 × 10−4

3.5

BC2-NbR106E

160 ± 0.48

12.0 ± 0.15

2.8 ± 0.01 × 105

3.4 ± 0.04 × 10−3

3.8

BC2-NbR106S

160 ± 0.43

9.7 ± 0.13

3.3 ± 0.03 × 105

3.2 ± 0.03 × 10−3

3.1

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