Figure 2 | Scientific Reports

Figure 2

From: Nested Machine Learning Facilitates Increased Sequence Content for Large-Scale Automated High Resolution Melt Genotyping

Figure 2

Melt curve shape diversity comparison between long and short amplicons of bacterial 16 S rRNA gene.

(A,B) Derivative melt curves for the long and short amplicons respectively from 29 organisms aligned at their -dF/dT value equal to 0.1 on the right side for visual shape comparison. (C,D) The average of all LAM curves in A and B respectively with the sample standard deviation at each point. Summation of standard deviations shows 1.75 times greater shape diversity (S.D. = 0.3169) for the long amplicon than the short amplicon (S.D. = 0.1816). (E) LAM curves of 29 organisms with only temperature calibrator alignment, showing smaller peak Tm range (1.75 °C). (F) Short amplicon melt curves of 29 organisms with only temperature calibrator alignment, showing a wider peak Tm range (2.5 °C, 1.43 times the long amplicon range).

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