Figure 4

LAM nested SVM identification tree.
Based on nested LOO SVM results of the training phase, the identification tree and testing phase SVM process is constructed. To test the identification of an experimental melt curve, round one SVM (left column) is used to classify the curve into either a group of organisms (middle column) or as an individual organism (right column). If the curve is identified to a group (middle column), it undergoes round two SVM within the group to be identified as an individual organism (right column). Middle column boxes correspond to groups that require further classification using SVM round two (Fig. 3). With this nested SVM process, all 37 organisms can be identified individually.