Figure 2
Gene expression changes in PBMCs in response to irradiation at 8 h after exposure.
In total, 125 genes were identified as differentially expressed by ANOVA (FDR corrected p-values < 0.05) between 0.1 Gy and 1.0 Gy samples and sham-irradiated controls. (a) Unsupervised hierarchical clustering analysis of these 125 genes (rows) showed good separation of samples (columns) depending on the dose of exposure. Red: high gene expression, blue: low gene expression. (b) 2D Principal Components Analysis (PCA) also separated samples depending on the dose. Each circle represents the expression profile of the 125 significantly differentially expressed genes in one sample. The percentage of the variance explained by the first and the second principal components is 44.7% and 6.58%, respectively. Ellipses represent two standard deviations. (c) Venn diagram showing the overlap of differentially expressed genes after exposure to 0.1 and 1.0 Gy of X-rays. (d) Unsupervised hierarchical clustering analysis of the 20 overlapping genes showed perfect separation of samples depending on the dose of exposure. Red: high gene expression, blue: low gene expression.
