Figure 2
From: Bioactivity of periodontal ligament stem cells on sodium titanate coated with graphene oxide
Characterization and flow cytometric analysis of human PDLSCs.
(a) Cell clusters derived from periodontal ligament formed a single colony and were stained with 0.1% toluidine blue. (b) After cultured in osteogenic induction media for 4 weeks, cells formed minerals positive for Alizarin Red staining. (c) After cultured in adipogenic induction media for 4 weeks, cells formed lipid vacuoles positive for Oil Red O staining. (d) PDLSCs were positive for MSCs markers CD44, CD73, CD90, CD105 and CD166 and negative for hematopoietic markers CD14, CD34 and CD45 (red line). Isotype controls 1B5 (IgG1) and 1D4.5 (IgG2a) were black line.
