Figure 2 | Scientific Reports

Figure 2

From: Downregulation of miR-522 suppresses proliferation and metastasis of non-small cell lung cancer cells by directly targeting DENN/MADD domain containing 2D

Figure 2

Effects of miR-522 on the proliferation of human NSCLC cells.

(a) Cells were transfected with miR-522 or miR-522 inhibitor and the expression of miR-522 was analyzed by qRT-PCR. *P < 0.05 vs miR-control. (b) The effects of miR-522 on NSCLC cells viability were measured with an MTT assay. A549 and H460 cells were transfected with miR-522 or miR-522 inhibitor for 24 h, 48 h and 72 h. All independent experiments were performed 3 times. *P < 0.05 vs miR-control. (c) The proliferation of A549 and H460 cells was determined by EdU kit. Nuclei that double labeled with EdU (green) and Hoechst 33342 (blue) were considered to be new proliferative cells, assessed by fluorescence microscopy (200×). *P < 0.05 vs miR-control.

Back to article page