Figure 6 | Scientific Reports

Figure 6

From: Downregulation of miR-522 suppresses proliferation and metastasis of non-small cell lung cancer cells by directly targeting DENN/MADD domain containing 2D

Figure 6

Identification of DENND2D as a miR-522 target gene.

(a) The potential miR-522 binding sites in the DENND2D 3′UTR and the mutated sequences. (b,c) 293T cells were co-transfected with miR-522, miR-522 inhibitor, NC or NC inhibitor with WT or Mut 3′UTR. The luciferase activity was assayed 48 h after transient co-transfection. *P < 0.05 vs miR-522. (d) The comparison of protein levels of DENND2D in tumors and adjacent normal tissues. (e) The mRNA expression level of DENND2D in NSCLC tissues and their corresponding adjacent tissues. *P < 0.05 vs Normal.

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