Figure 2
PS down-regulated expression of MMP-2 and -9 in HCECs exposed to hyperosmotic medium.
Primary HCECs were cultured in isomolar (312 mOsm) medium, then switched to hyperosmotic medium (450 mOsm) alone or in the presence of PS in different concentrations (5, 10 and 20 μM) for 4 h to evaluate mRNA level by RT-qPCR (A); or for 24 h to determine protein production and activity of MMP-2 and -9 by zymography (B). Data were summarized or representative from 3 separated experiments. *P < 0.05, as compared with 312 mOsM; vP < 0.05, as compared with 450 mOsM.
