Figure 3
PS blocked overproduction of reactive oxygen species (ROS) in HCECs exposed to hyperosmotic medium.
Primary HCECs were cultured in isomolar (312 mOsm) medium, then switched to hyperosmotic medium (450 mOsm) alone or in the presence of PS in different concentrations (5, 10 and 20 μM) for 3 h. ROS was analyzed with 2′, 7′-dichlorofluorescein diacetate (DCFDA) kit. (A) ROS production by HCECs in medium at 312 or 450 mOsM without or with 10 μM PS during 30–180 min, as measured by DCF fluorescence intensity. (B) ROS production suppressed by PS at different concentrations (5, 10, 20 μM) at 180 min. (C) The representative images showed positive cells of ROS-DCF fluorescence in HCECs at 450 mOsM for 120 min without or with 5–20 μM PS; the phase images showed equal cell density in 5 groups. Magnification 100x. Data were summarized or representative from 3 separated experiments. *P < 0.05, **P < 0.01, as compared with 312 mOsM; vP < 0.05, as compared with 450 mOsM.
