Figure 3
Unsecreted Wg induces ER stress in its expressing cells.
(a-a’) UAS-wgRNAi and UAS-miR-307a were co-expressed using enGal4. miR-307a-induced ectopic Bip staining was suppressed by wg knockdown (arrowhead in a). (b-b’) UAS-vps35RNAi was expressed in the A compartment using ciGal4. Ectopic Bip staining was observed in wg-expressing cells (b, arrowhead). (c-c’) vps26 RNAi was induced using hhGal4. Arrowhead in (c) indicates ectopic expression of Bip in the wg-expressing cells of P compartment. (d-d’) snx3 RNAi was induced using ciGal4. Arrowhead in (d) indicates ectopic expression of Bip in the wg-expressing cells of A compartment. (e-e”’) Knockdown of wg in a vps35 depletion background driven by ciGal4, the ectopic Bip staining was dramatically reduced (e”, arrowhead). (f-f”’) UAS-vps35RNAi and UAS-xbp1RNAi were co-expressed using enGal4. The ectopic Bip expression induced by vps35 knockdown was dramatically suppressed by xbp1 depletion (indicated by arrowhead in f”). (g-g”’) UAS-porcRNAi was expressed using ciGal4. Wg was accumulated in its expressing cells (g’). Bip was ectopically expressed in the wg-expressing cells (g”, arrow and arrowhead). Scale bar = 50 μm.
