Figure 6
COPI regulates Wg secretion and ER stress initiation.
(a) Wg staining in the wild-type wing disc. (a’) Enlarged view of the gray box region in (a). (b-b’) UAS-βCOPRNAi was expressed using C96Gal4. Wg secretion was disrupted by βCOP knockdown. The secreted punctate structures of Wg were dramatically reduced (indicated by arrows in b’). (c-c”’) Depletion of αCOP using C96Gal4 generated same phenotype (shown in c’). Ectopic Bip induction was also observed along the D/V boundary (shown in c”). (d) The ectopic Bip expression was suppressed by wg knockdown. (e-e”) UAS-wls and UAS-αCOPRNAi were co-expressed by C96Gal4 driver. αCOP depletion-induced Wg secretion defect cannot be rescued by wls overexpression (arrows in d’). (f) Adult wing of C96Gal4. (g-g’) UAS-αCOPRNAi or UAS-βCOPRNAi was expressed using C96Gal4. Knockdown either of them induced obvious wing notches (indicated by arrowheads in d’,d”). (h) Overexpression of wls failed to rescue the wing notching phenotype induced by αCOP knockdown. Scale bars in (a–e) 50 μm; (a’,b’,c’,e’) 20 μm.
