Figure 1
AZD3463 shows cytotoxic effects on NB cell lines.
(A) Six NB cell lines including the established chemoresistant NB cell line LA-N-6, were treated with the increasing concentrations of AZD3463 for 72 hrs. Cell viability was then assessed by adding a mixture of 10 μL of CCK-8 and 190 μL of RPMI and measuring absorbance at 450 nm. Data is represented as % vehicle ± S.D. with P < 0.05 (*), P < 0.01 (**), or P < 0.001 (***) (Student’s t-test, two-tailed) as indicated. (B) The IC50 values of AZD3463 in each cell line listed were calculated by using Prism 5, based on the data collected in the cell viability assay. ALK status in NB cell lines was also shown. (C) Morphological changes of six different NB cell lines treated with increasing concentrations of AZD3463 for 72 hrs were shown. (D) PI staining of IMR-32, SH-SY5Y, NGP and SK-N-AS cells with the indicated concentrations of AZD3463 for 0, 2 and 4 hrs were shown. The cells were then analyzed by flow cytometry for the percentage of dead cells by incubating with PI for 15 min after AZD3463 treatment.
