Figure 2

AZD3463 suppresses anchorage-independent growth of NB cells.
(A) A panel of six NB cell lines were seeded in six-well plates with indicated concentrations of AZD3463 and agar and grown for 2 to 3 weeks. Cells were stained with crystal violet for 4 hrs and images were obtained. (B) Colonies were counted and colony numbers were represented as % vehicle ± S.D. with P < 0.05 (*), P < 0.01 (**) or P < 0.001 (***) (Student’s t-test, two-tailed) as indicated.