Figure 4 | Scientific Reports

Figure 4

From: Novel ALK inhibitor AZD3463 inhibits neuroblastoma growth by overcoming crizotinib resistance and inducing apoptosis

Figure 4

AZD3463 enhances the cytotoxic effect of Dox on NB cell lines.

(A) IMR-32, NGP, SH-SY5Y, SK-N-AS and LA-N-6 cells were seeded in 96-well plates and were incubated with the indicated concentrations of Dox plus DMSO or 1 μM of AZD3463 for 48 hrs. Cell viability was then measured by CCK-8 assay. (B) IMR-32, NGP, SH-SY5Y, SK-N-AS and LA-N-6 cells were treated with either Dox (1 μM) alone, AZD3463 (1 μM) alone, or a combination for varying durations (0–8 hrs or 0–16 hrs), subjected to SDS-PAGE and immunoblotted with PARP and Caspase-3 antibodies. β-Actin antibodies were used as a loading control for whole cell extracts in all samples.

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