Table 3 Single nucleotide polymorphism (SNP) in a grazing suppressed gene annotated by three GO biological process terms: response to hypoxia, response to decreased oxygen levels, and response to oxygen levels.

From: Transcriptome responses in alfalfa associated with tolerance to intensive animal grazing

   

Tolerant plants

Intolerant plants

Amino acid

MF200401 (grazed)

MF200401-1 (not grazed)

MF200402 (grazed)

change

Unigene ID

Pos from 5′ end

Base

SNP

Base

SNP

Base

SNP

Base

 

Medicago truncatula isoflavone 2′-hydroxylase

CL5196.Contig1_All

1604

G

—

G

—

G

K

G:T

3′ UTR

  1. Homologous transcripts of this gene were suppressed by grazing in the MF200401 tolerant plants, and were expressed at even lower levels in the grazed MF200402 intolerant plants. A SNP was reported for transcripts that were monomorphic between the transcriptomes of the grazed and ungrazed tolerant plants, but polymorphic with respect to the transcriptome of the intolerant plants. Pos is the position of the SNP on the assembled transcript from the 5′ end based on sequence data from all samples. Base is the consensus nucleotide observed across all three alfalfa transcriptomes evaluated. SNP is the IUPAC code for nucleotide variation observed at a given position. Amino acid change is obtained by aligning the SNP with coding sequences of known proteins. If the SNP is not located on any coding sequences, it is annotated by either 5′ or 3′ untranslated region (UTR).
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