Table 5 Single nucleotide polymorphism (SNP) in five grazing upregulated genes involved in three metabolic pathways.

From: Transcriptome responses in alfalfa associated with tolerance to intensive animal grazing

Unigene ID

Pos from 5′ end

Base

Tolerant plants

Intolerant plants

Amino acid

MF200401 (grazed)

MF200401-1 (not grazed)

MF200402 (grazed)

change

SNP

Base

SNP

Base

SNP

Base

 

Pathway ko01110 Biosynthesis of secondary metabolites:

K01850 chorismate mutase [EC:5.4.99.5]

CL10594.Contig2_All

369

A

—

A

—

A

R

G:A

Synonymous

Unigene16098_All

456

A

—

A

R

A:G

W

A:T

Asn→Ile (T);Asn→Ser (G)

K15405 cytochrome P450, family 96, subfamily A, polypeptide 15 (midchain alkane hydroxylase)

CL1941.Contig2_All

451

A

W

A:T

—

A

W

A:T

Synonymous

K12373 hexosaminidase [EC:3.2.1.52]

CL4571.Contig3_All

647

G

R

G:A

R

G:A

R

G:A

Synonymous

K00869 mevalonate kinase [EC:2.7.1.36]

Unigene11434_All

213

C

Y

C:T

—

C

Y

C:T

5′ UTR

Unigene19167_All

476

T

K

T:G

—

T

K

G:T

3′ UTR

K13789 geranylgeranyl diphosphate synthase, type II [EC:2.5.1.1 2.5.1.10 2.5.1.29]

Unigene4300_All

174

C

—

C

—

C

M

C:A

Ser→Tyr

Pathway ko00511 Other glycan degradation:

K12373 hexosaminidase [EC:3.2.1.52]

CL4571.Contig3_All

647

G

R

G:A

R

G:A

R

G:A

Synonymous

Pathway ko00073 Cutin, suberine and wax biosynthesis:

K15405 cytochrome P450, family 96, subfamily A, polypeptide 15 (midchain alkane hydroxylase)

CL1941.Contig2_All

451

A

W

A:T

—

A

W

A:T

Synonymous

  1. Homologous transcripts of these genes were upregulated by grazing in the MF200401 tolerant population and were also more abundantly expressed in the tolerant versus intolerant MF200402 plants, both of which were grazed. A SNP was reported for transcripts that were monomorphic between the transcriptomes of the grazed and ungrazed tolerant plants, but polymorphic with respect to the transcriptome of the intolerant plants. Pos is the position of the SNP on the assembled transcript from the 5′ end based on sequence data from all samples. Base is the consensus nucleotide observed across all three alfalfa transcriptomes evaluated. SNP is the IUPAC code for nucleotide variation observed at a given position. Amino acid change is obtained by aligning the SNP with coding sequences of known proteins. If the SNP is not located on any coding sequences, it is annotated by either 5′ or 3′ untranslated region (UTR).