Figure 2
From: De-repression of CSP-1 activates adaptive responses to antifungal azoles
Overexpression of csp-1 increases azole sensitivity.
(A) Transcripts of csp-1 detected by quantitative real-time polymerase chain reaction. The transcriptional increase of csp-1 was calculated relative to the transcript level in the WT strain. Values shown are means of three replicates. Standard deviations are indicated with error bars. (B) Azole sensitivity test. Conidial suspensions of the WT and csp-1 overexpression strain (csp-1OE) were inoculated onto solid medium, with or without azoles and incubated at 28 °C in the dark. Images were captured at 24 h for the control and at 48 h for the azole-treated plates (at 24 h, none of the tested strains formed colonies on the plates containing azoles). (C) Relative inhibition rates. The calculation followed the method described in Fig. 1B. pket = 0.00005, n = 3; pvori = 0.0005, n = 3.
