Table 1 Luciferase assay results of tested fragments in two cell lines, presented as the mean ratio of the constructs to pTK (a basic promoter vector), together with one standard deviation.

From: Transcriptional regulation of PRPF31 gene expression by MSR1 repeat elements causes incomplete penetrance in retinitis pigmentosa

 

Fold induction over pTK

HeLa

RPE-1

BiP-MSR-3x

0.20 ± 0.07

0.07 ± 0.03

BiP-MSR-4x

10.63 ± 1.63

8.05 ± 1.36

pTK-MSR-2x (+)

2.85 ± 0.49

4.53 ± 0.64

pTK-MSR-3x (+)

1.27 ± 0.26

3.11 ± 0.35

pTK-MSR-4x (+)

0.56 ± 0.16

1.96 ± 0.24

pTK-MSR-2x (−)

2.05 ± 0.49

3.45 ± 0.36

pTK-MSR-3x (−)

1.14 ± 0.20

1.78 ± 0.24

pTK-MSR-4x (−)

0.79 ± 0.10

0.97 ± 0.19

Δ1-MSR-2x

0.042 ± 0.012

0.065 ± 0.013

Δ1-MSR-3x

0.041 ± 0.010

0.069 ± 0.012

Δ1-MSR-4x

0.040 ± 0.009

0.086 ± 0.024

pTK (positive control)

1.00 ± 0.06

1.00 ± 0.14

pGL3 (negative control)

0.008 ± 0.004

0.074 ± 0.023

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