Figure 2
Schematic diagram of the experimental design comparing the comparator12 and PureLyse® sample preparation methods.
TB-negative pooled sputum (~1 mL) samples were spiked with 105, 104, 103, or 0 cfu M.tb H37Ra and processed by both methods. Extracted nucleic acids were then amplified and detected using qPCR, as described by Halse et al.12.
