Figure 3
qPCR detection of M.tb H37Ra spiked into sputum, extracted via the comparator (black diamond) and PureLyse® methods (white square): mean and standard deviation from six biological replicates, with two technical replicates each.
At 105 and 104 cfu/mL, all replicate samples amplified. At 103 cfu/mL, four of six samples and two of six samples amplified for the comparator and PureLyse® methods, respectively.
