Figure 5
From: DNMT3L enables accumulation and inheritance of epimutations in transgenic Drosophila
Misregulation of gene expression in flies expressing DNMT3L is gradual and progressive.
(A–F) Scatter plots showing the upregulated and downregulated genes in each generation of the DNMT3L expressing transgenic flies. Comparison was made between gene expression levels in 3rd instar Tub-3L larvae from a particular generation (indicated on the Y Axis) with UAS-3L control larvae from G1. Note a progressive increase in the number of genes that were aberrantly expressed in successive generations. Green crosses represent upregulated genes while red crosses represent down regulated genes. Number of genes misregulated was minimal in G20 flies that lacked DNMT3L expression due to the removal of the GAL4 driver. G1, G2, G4, G5, denotes Tub-3L larvae from the particular generation. G5P denotes 5th generation Tub-3L larvae with melanotic tumors. G20*-UAS-3L*- denotes G20 flies after removal of the GAL4 driver by crossing flies with double balancers.
