Figure 6

Nuclear reprogramming in DNMT3L expressing Drosophila: Microarray data analysis.

(A) Heat map depicting hierarchical clustering of differentially expressed genes in the various samples and examined by microarray analysis. The heat map shows the relative expression levels of each transcript in each samples. Green denotes downregulated, red denotes upregulated and black depicts genes that showed no change with respect to their expression level in control G1 UAS-DNMT3L transgenic larvae. Note the similarity in the heat map profile of the biological replicates for each generation. Sample numbers given above the profile denote: are as follows 1- G1-UAS-3L; 2 to 5 –Tub-3L larvae from generation G1, G2, G4 and G5; 6 – G5-Tub-3L larvae with tumors, 7 – G5-UAS-3L and 8- G20*-UAS-3L* larvae from 20^th generation where GAL4 driver was removed. (B,C) Venn diagrams depicting genes upregulated (B) and downregulated (C) in DNMT3L expressing flies across various generations. The number of genes that remain misregulated in successive generations increased progressively (see also Supplementary Table 4). G1 to G5 depict generation number, G5Phen denotes genes misregulated in G5 Tub-3L larvae that had melanotic tumors.