Figure 8
From: DNMT3L enables accumulation and inheritance of epimutations in transgenic Drosophila
Accumulation of aberrant histone methylation in DNMT3L expressing Drosophila across successive generations.
(A) Western blot analysis demonstrating decrease in the levels of the H3K4me3 in 3rd instar larvae or adult flies expressing DNMT3L from the 5th generation as compared to control UAS-3L flies lacking the GAL4 driver. Histone H3 was used as loading control. (B) Immunostaining of Drosophila polytene chromosomes with H3K4me3 antibody. (C) Western blot analysis for the various histone modification as indicated, performed on larvae from the various generations of control and DNMT3L expressing Drosophila larvae. The densitometric scan values for each signal was calculated and is represented graphically in (D) as fold change with respect to the level of the respective histone modification in G1-UAS-3L flies lacking GAL4 driver. G1 and G5-UAS-3L are control larvae without GAL4 driver from G1 and G5 generation respectively. G1 to G5- Tub-3L larvae from the indicated generation. G5P- G5 Tub-3L larvae that had melanotic tumors. G20-UAS-3L* denotes larvae from G20 generation after crossing out of the Tubulin-GAL4 driver. Actin was used as a loading control. The error bars represent Standard Deviation (S.D.). *Indicate significant difference (Student’s t test, *p < 0.05, **p < 0.01).
