Figure 9
From: DNMT3L enables accumulation and inheritance of epimutations in transgenic Drosophila
ChIP analysis for association of DNMT3L and specific Histone modifcations with specific gene promoters.
Graphical representation of the DNMT3L binding (A), association of H3K4me3 (B) and H3K36me3 (C) with the promoter and HeK36me3 within the exonic regions (D) of selected upregulated, downregulated and unaffected genes tested by ChIP using specific antibodies. Results are represented as % input. The names of the specific gene promoters tested are given below the X-axis. U1- control larvae without GAL4 driver from the 1st generation G1, G3 and G5- Tub-3L larvae from the indicated generation. G5P denotes 5th generation Tub-3L larvae having melanotic tumors. G20* denotes larvae from G20 generation after crossing out of the Tubulin-GAL4 driver. The error bars represent Standard Deviation (S.D.). *Indicate significant difference (Student’s t test, *p < 0.05, **p < 0.01).
