Table 2 Melanotic tumors are not observed after the removal of Tubulin-GAL4 driver.

From: DNMT3L enables accumulation and inheritance of epimutations in transgenic Drosophila

Generation No.

Crosses

No. of larvae with tumors/Total

No. of Larvae

Set1

Set2

Set3

G21

G20(Tub-3L) ♀ X db ♂

0/118

0/116

0/108

db♀ X G20(Tub-3L) ♂

0/116

0/112

0/121

G22

G21 (UAS-3L*) ♀ X db ♂

0/111

0/96

0/101

db ♀X G21(UAS-3L*) ♂

0/98

0/106

0/110

G23

G22 (UAS-3L*)♀ X db ♂

0/101

0/102

0/95

db ♀X G22(UAS-3L*) ♂

0/103

0/105

0/97

G24

G23(UAS-3L*)♀ X db ♂

0/103

0/107

0/109

db ♀ X G23(UAS-3L*) ♂

0/122

0/110

0/113

G25

G24(UAS-3L*)♀ X db ♂

0/108

0/98

0/101

db ♀ X G24(UAS-3L*) ♂

0/107

0/89

0/104

  1. Tub-3L- DNMT3L expressing flies with Tubulin-GAL4 driver; db- Double balancer flies (Pin/CyO; Tm2/Tm6); UAS-3L* – DNMT3L flies after removal of the Tubulin-GAL4 driver.