Figure 5
From: Interaction between IGFBP7 and insulin: a theoretical and experimental study
Pull-down assay of IGFBP7 mutants with insulin and IGFBP7 antibody. His6-tagged-IGFBP7 (0.1 mg, wild-type and mutants) were mixed with 0.5 mg insulin.
The mixture was loaded on to a Ni-Sepharose 6 column. The eluted materials were subjected to SDS-PAGE and Western blotting with the anti–insulin antibody (upper panel) or the anti–IGFBP7 antibody (lower panel). Lanes from left to right, wild-type IGFBP7, R198I-H200F mutant, in triplicate. All bands shown are representative of at least three separate experiments, with error bars representing SD. The graph shows densitometric analysis of bands.
