Figure 4
THSD7A mediates the invasion-inhibitory effect of miR-210 in HTR8/SVneo cells.
(a). Anti-miR-210 decreased miR-210 levels. HTR8/SVneo cells were transfected with anti-miR-210 or its non-targeting control (anti-NC) and miR-210 levels were measured at 48 hours after transfection by qRT-PCR. The level of miR-210 was adjusted by the value of U6 and the relative value was presented as the mean ± SEM based on three independent experiments. **P < 0.01 vs NC. (b). Confirmation of siRNA-mediated knockdown of THSD7A. HTR8/SVneo cells were transfected with THSD7A specific siRNA (si-THSD7A) or its scramble control (NC). The level of THSD7A was determined by qRT-PCR and normalized to GAPDH. Data represent mean ± SEM of three independent experiments. **P < 0.01 vs NC. c, d. Typical result (c) and statistical result (d) of transwell invasion assays. HTR8/SVneo cells were transfected with miR-210 mimics, NC, anti-miR-210 and /or specific siRNA for THSD7A. The invasion indices were presented as the mean ± SEM based on three independent experiments. * and ** compared with the corresponding control, P < 0.05 and P < 0.01, respectively.
