Figure 2
From: Secreted Thrombospondin-1 Regulates Macrophage Interleukin-1β Production and Activation through CD47
Soluble TSP1 in the presence of LPS causes IL-1β maturation.
(A) Differentiated THP-1 were incubated with soluble TSP1 (10, 30, 100, 300 ng/ml). (B) Primary monocyte derived macrophages were incubated with soluble TSP1 (0.1, 1, 10, 100 ng/ml). After 15-20 min either medium or 20 ng/ml LPS was added for an additional 3 h. Medium or 5 mM ATP was added in the final 30 min where indicated. Supernatant (sup) and cell lysates (lys) from each experiment were collected and analyzed by western blot for caspase-1, IL-1β and β-actin. (C) Supernatant was analyzed for total secreted human IL-1β using ELISA. In panel B significance was calculated using one-way parametric ANOVA. When data significance is indicated “*” means p < 0.05, “**” means p < 0.01 and “***” means p < 0.005. Data shown is a representative of 3 independent experiments performed.
