Figure 3
From: Secreted Thrombospondin-1 Regulates Macrophage Interleukin-1β Production and Activation through CD47
Wild type BMDMs have significantly more caspase-1 and IL-1β mRNA than cd47−/− bone marrow derived macrophages after LPS stimulation.
(A) Graph depicts basal levels of IL-1β, NLRP3 and caspase-1 in wild type and cd47−/− mRNAs following twenty separate experiments. (B–D) Cells were stimulated with LPS as indicated for 3 h. 2.5 mM ATP was added to wells for the final 30 min. Levels of IL-1β, NLRP3 and caspase-1 mRNA were measured using quantitative real-time PCR. In panel (A) significance was calculated using two-way ANOVA. In panel (B) significance was calculated using unpaired Student’s t-test. When data significance is indicated “*” means p < 0.05, “**” means p < 0.01 and “***” means p < 0.005. Data shown is a representative of 3 independent experiments performed.
