Figure 6
From: Secreted Thrombospondin-1 Regulates Macrophage Interleukin-1β Production and Activation through CD47
TSP1-driven IL-1β maturation is CD47-dependent.
(A) Differentiated THP-1 cells were cultured with a mouse anti-human CD47 (B6H12) monoclonal antibody or IgG k anti-mouse isotype control for 15 min. Soluble TSP1 was added at increasing concentrations and after 15 min, 20 ng/ml LPS was added. (B) THP-1 monocyte derived macrophages were treated with 22 pM of recombinant TSP1 domains: (3TSR, NoC1, or E123CaG1) for 15 min and then either LPS or medium was added to the cells for 3 hours. sup = conditioned medium from the well following experiments; lysate = cell lysis buffer was added to wells to assess intracellular protein levels. Data shown are representative of 3 experiments performed.
