Table 1 Strains and plasmids used in this study.
Strain or plasmid | Genotype/Description | Reference |
|---|---|---|
Strains | ||
DH10B | F−endA1 recA1 galE15 galK16 nupGrpsLΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrAΔ(mrr-hsdRMS-mcrBC) λ− | |
AB1157 | thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr′-0, glnV44(AS), galK2(Oc), LAM-, Rac-0, hisG4(Oc), rfbC1, mgl-51, rpoS396(Am), rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), thi-1 | |
AB1157 xthA−/nfo− | As AB1157 butnfo::Kan, Δ(xthA-pncA)DE | CNRS |
BL21 (DE3) pLysS | F- ompT gal dcmlonhsdSB(rB- mB-) λ(DE3) pLysS(cmR) | Promega |
Plasmids | Description | |
pBC SK | Cloning vector, Camr | Stratagene |
pBC-41 F | pBC vector plus 1.5 kb environmental insert | This study |
pCR-Blunt II-TOPO | Cloning vector ,Kanr | Invitrogen |
pCR-Blunt-EXOMEG1 | pCR-Blunt vector plus 975 pb environmental ORF | This study |
pHisParallel | Expression vector, IPTG inducible, Ampr | |
His-EXOMEG1 | pHis vector plus 975 pb environmental gene | This study |
pTrc99A | Cloning vector, IPTG inducible, Ampr, it allows T7 polymerase-independent expression | |
pTrc-Exomeg1 | pTrc99A plus 975 pb environmental gene | This study |
