Figure 1
From: TCTP contains a BH3-like domain, which instead of inhibiting, activates Bcl-xL
TCTP contains a non-canonical BH3 domain interacting with the BH3 groove of Bcl-xL.
(A) Sequence alignment of the N-terminal region of TCTP (h:human; m:mouse; d:Drosophila; y:yeast; p:plant) with other BH3 domain containing proteins. In red, the conserved hydrophobic residues of the BH3 domain (named h1 (P1), h2 (P2), h3 (P3) and h4 (P4)). In green, the GD signature of BH3 domains. In blue, the conserved charged residues. (B) Interaction between Bcl-xL and TCTP-BH3 peptides. Calorimetric titration between (left panel) Bcl-xL (0.065 mM) and increasing amount of TCTP-BH3 (1.39 mM); (middle panel) Bcl-xL-Y101K (0.072 mM) and increasing amount of TCTP-BH3 (0.955 mM); (right panel) Bcl-xL (0.065 mM) and increasing amount of TCTP-BH3-R21A (1.26 mM). Each experiment was carried out at 30 °C in 50 mM ammonium bicarbonate pH 9. (C) In cellulo BRET analysis between TCTP and Bcl-xL, inhibition by ABT-737, lack of binding for the R21A TCTP to Bcl-xL (left panel) and Bax (right panel).
