Figure 3
In-gel detection of cytochrome c oxidase activity in BN-gels run with solubilised membranes of S. oneidensis.
Membrane proteins were prepared from S. oneidensis wild-type (a,c) and strain SLL01 lacking the cbb3-type oxidase (b,d) grown in aerobic (a,b) or highly aerobic (c,d) conditions. The S. oneidensis strains were grown on rich medium (LB), minimal medium (MM3), iron-depleted medium (MM3I−) or carbon-depleted medium (MM3C−) and cells were harvested during the exponential (E) or stationary (S) phase of growth. Total proteins (130 μg) were loaded on a 5–15% polyacrylamide gel. Bands of activity are indicated by roman numerals. Molecular mass markers are indicated in kDa.
