Figure 1
From: Generating trunk neural crest from human pluripotent stem cells
Blockade of BMP signaling is not required for NCC differentiation and promotes upregulation of CNS-related markers.
(A,B) H1 ESC were differentiated toward NCC via protocols published by the Dalton or Studer lab. At the end of each protocol, expression of NCC markers (A) or CNS-related markers (B) was analyzed using RT-qPCR and compared against the expression of these markers in human dermal fibroblasts (HDF). Dalton protocol showed higher levels of NCC markers, while suppressing CNS-related markers compared to the Studer protocol. (C) Immunofluorescence analysis of Dalton protocol-derived NCC for NCC markers HNK1/p75 (top) and FOXD3/SOX10 (bottom). *p < 0.05, **p < 0.01, ***p < 0.001. Scale bars represent 50 μm.
