Figure 3
From: Generating trunk neural crest from human pluripotent stem cells
RA treatment upregulates multiple HOX genes and alters the differentiation potential of NCC.
(A,B) Trunk NCC were analyzed via RT-qPCR for (A) HOXA, HOXB, (B) HOXC and HOXD genes previously described to be detected in trunk NCC and were found to be upregulated compared to cranial NCC (not treated with RA). All genes are expressed at statistically significant levels (p < 0.05) in trunk NCC compared to cranial NCC except for HOXB7 (n.s. = not significant) (C) Trunk NCC were compared against cranial NCC for NCC progenitor markers PHOX2B (sympathoadrenal), MITF (melanoblasts), S100β (Schwann cells) and TUBB3 (neurons). ***p < 0.001.
