Figure 5 | Scientific Reports

Figure 5

From: Loss of CAR promotes migration and proliferation of HaCaT cells and accelerates wound healing in rats via Src-p38 MAPK pathway

Figure 5

Study to assess the effect of PP2 or SB203580 pretreatment on CAR knockdown-induced acceleration of cell migration and proliferation in HaCaT cells.

(A) HaCaT cells were pretreated with vehicle (DMSO), PP2 or SB203580 (a p38 inhibitor) for 2 h before RNAi. Before scratch wound was made, cells received 1-h mitomycin C treatment to inhibit cell proliferation. The width of each scratch gap was monitored at 0, 24, 48 and 60 h post-scratching. Blunted lines indicate the average width of unclosed gap. Scale bar:100 μm. (B) Scratch gap at 0 h in each treatment group was arbitrarily set at 1. Error bars represent means ± SD from four independent experiments using different batches of cells (n = 4), *p < 0.05. (C) MTT assay was conducted after cells receiving drug and RNAi treatments at designated time points of 0, 12, 24, 36 and 48h. Error bars represent means ± SD from four independent experiments using different batches of cells (n = 4), *p < 0.05. ‘Scr’, scramble; ‘Veh’, vehicle.

Back to article page