Figure 3
From: The role of PD-L1 in the radiation response and clinical outcome for bladder cancer
PD-L1 linked with the radiation response of murine bladder cancer in vivo.
The effects of irradiation on PD-L1 expression in vivo were evaluated by (a) FACS and (b) IHC analyses using murine bladder tumour specimens at the indicated times following 12 Gy irradiation. Furthermore, effects of PD-L1 blockade on radiosensitivity in vivo were evaluated as the (c) growth delay of irradiated bladder tumours. The Y- axis shows the ratio of tumour volumes at each time point divided by that at irradiation, respectively. Each point is shown as the means of 3 separate experiments; bars, SD. *P < 0.05; (d) using an orthotopic murine tumour model, IHC for tumour cell proliferation rate at 7 days after irradiation; immunofluorescence for apoptosis at 72 h after irradiation; and western blot analysis for ki-67 and cleaved caspase 3 in tumours at indicated time after irradiation. Representative slides are shown.
