Figure 4
From: The role of PD-L1 in the radiation response and clinical outcome for bladder cancer
Correlation between irradiation, PD-L1 and cytotoxic T cell accumulation.
The effect of PD-L1 blockade on tumour-infiltrated cytotoxic T cells was evaluated by (a) FACS using CD3-CD8 and (b) immunofluorescence analysis (DAPI, blue; CD3, Red; and CD8, Green) using irradiated tumour specimens. (c) The effect of CD8+ T cells on PD-L1 Ab-induced radiosensitization was further evaluated in irradiated mice treated with or without CD-8 neutralizing antibodies using tumour growth delay after irradiation. (d) The effect of PD-L1 blockade on the suppressing ability of tumor cells for T cells proliferation was evaluated by FACS. Quantitative data are shown. (e) The effect of PD-L1 blockade on the CD8+ T cells cytotoxicity against irradiated cancer cells was evaluated by FACS. Representative images and quantitative data are shown.
