Figure 1
iPSC microvesicle diameter and release rate.
(A–C) MV nanometer diameter range and concentration were derived from iPSC conditioned media at 12 h, 24 h and 48 h, respectively. MV diameters and concentrations for three samples at each time point are indicated in colored traces. Mean diameters and standard deviation for each time point remained consistent; 12 h 122 ± 2.3 nm, 24 h 124 ±6.0 nm, 48 h 122 ± 2.2 nm. (D) Arrows show light-scattering of individual MVs from a single frame of Nanosight tracking analysis at 24 h. (E) 3D plot showing MV size/relative intensity. (F) Average number of MVs in conditioned media at 12 h = (1.69 ± 0.66)* 108, 24 h = (3.80 ± 0.77)* 108 and 48 h = (4.20 ± 0.40)* 108. Error bars represent standard deviation. Student’s t-test was performed to analyze iPSC release rates. p < 0.05 was considered statistically significant. “ns” represents not significantly different.
