Figure 2

Analysis of cell surface microvesicle formation.
(A) TEM image of exosome formation at the iPSC surface (Fig. 2A, arrowhead), scale: 500 nm. (B) Bright field image of iPSC with surface topology suggestive of vesicle formation at the cell membrane, (C) Following PKH26 (red) lipophilic labeling, emerging vesicles at the iPSC lipid bilayer appear concentrated near the soma with observable variations in size, (d) DAPI nuclear labeling with overlay of (B,C), scale: 10 μm.