Figure 4
iPSC microvesicles contain pluripotent transcription factor mRNA.
(A) Total RNA isolated from iPSC derived MVs lacks 28 S and 18 S rRNA as revealed through 2% denaturing agarose gel loaded with total RNA from iPSC MVs and iPSCs. (B) To verify the presence of selected iPSC derived mRNA, MV mRNA was analyzed using real-time PCR and contained factors involved in the maintenance of iPSC pluripotency including Oct-3/4, Nanog, Klf4 and C-Myc, Positive controls β- actin and GAPDH were present in iPSCs and MVs. Significant differences between cells and MVs are denoted with asterisks: *(p < 0.05), **(p < 0.01), ***(p < 0.001), ****(p < 0.0001); “ns” - not significantly different.
