Figure 5

Immunogold EM analysis of Microvesicle Protein Content.
Immunogold EM images reveal the presence of (A) the homeodomain transcription factor Nanog, (B) the homeodomain transcription factor Oct-3/4, (C) the tetraspanprotein Tsg101, a canonical MV marker and (D) control. Each pluripotent protein identified within MVs is involved in undifferentiated iPSC self-renewal. Samples were viewed under a FeiTecnai Transmission Electron Microscope. Scale: 50 nm.