Table 1 Primers used for real-time PCR.
Primer pairs | Sequence (5′–3′) | Amplicon (bp) |
|---|---|---|
C-Myc | CAGAGGAGGAACGAGCTGAAGCGC | 228 bp |
TTATGCACCAGAGTTTCGAAGCTGTTCG | ||
Nanog | AGGGTCTGCTACTGAGA TGCTCTG | 228 bp |
CAACCACTGGTTTTTCTGCCACCG | ||
Oct-3/4 | CTGAGGGCCAGGCAGGAGCACGAG | 485 bp |
CTGTAGGGAGGGCTTCGGGCACTT | ||
Klf4 | CACCATGGACCCGGGCGTGGCTGCCAGAAA | 739 bp |
TTAGGCTGTTCTTTTCCGGGGCCACGA | ||
β- actin | TGTTACCAACTGGGACGACA | 150 bp |
ACCTGGGTCATCTTTTCACG | ||
GAPDH | TGGCAAAGTGGAGATTGTTGCC | 150 bp |
AAGATGGTGATGGGCTTCCCG |
