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Figure 2

From: Autologous skeletal muscle derived cells expressing a novel functional dystrophin provide a potential therapy for Duchenne Muscular Dystrophy

Figure 2

Expression of dystrophin in lentivirally-transduced pericytes.

(A) Confocal images of DMD pericytes that had been transduced with dystrophin lentivirus. GFP+ cells were FACS sorted and plated in culture, then fixed with 4% paraformaldehyde and stained with GFP (green) and dystrophin (red). Nuclei were counterstained with DAPI (blue). Scale bar = 20 μm. SC1: SFFV-C1-GFP, DC1: hDesmin-C1-GFP, SC2: SFFV-C2-GFP and DC2: hDesmin-C2-GFP. (B) Western blotting of GFP (upper lane) and Tubulin 2.1 (lower lane) on samples extracted from DMD pericytes which had been transduced with SFFV-C1-GFP (SC1); hDesmin-C1-GFP (DC1); SFFV-C2-GFP (SC2) and hDesmin-C2-GFP (DC2) lentivirus. GFP+ cells were purified by FACS before sample preparation. Sample extracted from non-transduced (NT) pericytes was loaded as negative control. This is a cropped image; the full-length blot is presented in Supplementary Figure S2.

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