Figure 3 | Scientific Reports

Figure 3

From: Autologous skeletal muscle derived cells expressing a novel functional dystrophin provide a potential therapy for Duchenne Muscular Dystrophy

Figure 3

Expression of dystrophin in differentiating pericytes that had been transduced with SFFV-C1-GFP (SC1); hDesmin-C1-GFP (DC1); SFFV-C2-GFP (SC2) and hDesmin-C2-GFP (DC2) lentivirus.

Purified GFP+ cells from each cell preparation were induced to differentiate in culture for 7 days and stained with antibodies to GFP (green), dystrophin (red) and myosin (MF20, cyan). hCD133+ cells derived from a normal donor were treated in parallel as positive control for the dystrophin staining. White arrows point to cells that express GFP and dystrophin, but did not express myosin and were not differentiated multinucleated myotubes. Nuclei were counterstained with DAPI (blue). Scale bar = 15 μm.

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