Figure 4 | Scientific Reports

Figure 4

From: Autologous skeletal muscle derived cells expressing a novel functional dystrophin provide a potential therapy for Duchenne Muscular Dystrophy

Figure 4

Restoration of dystrophin in regenerated muscle fibres derived from lentivirally-transduced DMD pericytes.

(A) shows representative transverse sections of cryodamaged muscles of an mdx nude mouse that had been grafted with a–d: SFFV-C1-GFP cells; e–h: hDesmin-C1-GFP cells. Muscles transplanted with SFFV-C2-GFP and hDesmin-C2-GFP cells showed similar staining pattern as that shown in a–d. Sections were stained with antibodies to human spectrin (red), GFP (green), dystrophin (cyan). Nuclei were counterstained with DAPI (blue). Scale bar = 15 μm. (B) and (C) are the quantification of human Spectrin+ fibres (B) and GFP+ fibres (C) in SFFV-C1-GFP, hDesmin-C1-GFP, SFFV-C2-GFP and hDesmin-C2-GFP cells transplanted groups. *p < 0.05.

Back to article page