Figure 5 | Scientific Reports

Figure 5

From: Autologous skeletal muscle derived cells expressing a novel functional dystrophin provide a potential therapy for Duchenne Muscular Dystrophy

Figure 5

Recruitment of members of the DGC in dystrophin-expressing fibres of donor origin.

Representative transverse sections of muscles that had been transplanted with SFFV-C1-GFP donor cells, stained with antibodies to: GFP (green, a, f, k), α-sarcoglycan (α-SG, red, b), β-dystroglycan (β-DG, red, g) and γ-sarcoglycan (γ-SG, red, l). c, h and m are merged images of a and b; f and g; k and l, respectively. Nuclei were counterstained with DAPI (blue). Scale bar = 15 μm. Muscles that had been transplanted with SFFV-C2-GFP, hDesmin-C2-GFP cells (not shown here) showed a similar staining pattern to SFFV-C1-GFP cell transplanted muscles. d, i and n: expression intensity of α-sarcoglycan (d), β-dystroglycan (i) and γ-sarcoglycan (n) was significantly higher in GFP+ compared to GFP- fibres in representative muscles that had been transplanted with SFFV-C1-GFP, SFFV-C2-GFP and hDesmin-C2-GFP cells. The intensity of expression of each component of the DGC correlated positively to GFP intensity (e, j and o). ***p < 0.0001.

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