Figure 5
From: Essential role of proteasomes in maintaining self-renewal in neural progenitor cells
Suppressed proliferation and neuronal differentiation of cultured NPCs following PSMB5 knockdown-triggered decrease in proteasomal activity.
(A) NPCs isolated from E14 mice were transfected with si-PSMB5 for 72 hrs. The knockdown efficiency was measured by qPCR (a) and western blotting (b). (c) PSMB5 knockdown reduced proteasomal activity in NPCs. (B) PSMB5 knockdown prevented the neurosphere formation, as indicated by decreases in both the number and diameter of neurospheres. (C) The decline of dividing cells was found in NPCs transfected with si-PSMB5 by BrdU incorporation (a). (b) Levels of the cell cycle-related proteins Cyclin D1 and CDK4 were lowered in NPCs treated with si-PSMB5. (D) NPCs transfected with either scramble siRNA or si-PSMB5 were cultured in the differentiation medium for 5 days. Neuronal differentiation was evaluated by the Tuj1/DAPI percentage. *p < 0.05 and **p < 0.01 vs. Scramble control.
