Figure 6
From: A novel type bacterial flagellar motor that can use divalent cations as a coupling ion
Effect of Mg2+, protonophore CCCP and Na + channel inhibitor EIPA on swimming speed of B. subtilis mutant strains.
BS-AB, BS-PS, OF4PS, TCA-AB1 and TCA-AB2 mutant strains were grown for 6 h at 37 °C in Spizizen I medium plus 1 mM MgCl2 and 1% xylose at pH 8.0 with shaking. Cells were suspended in 1 ml of phosphate buffer (pH 8.0) (A), plus the indicated amounts of CCCP plus 10 mM MgCl2 (B), plus the indicated amounts of EIPA plus 10 mM MgCl2 and 100 mM NaCl (C) and then incubated at 37 °C for 10 min. Phosphate buffer contained 10 mM potassium phosphate (pH 8.0), 5 mM glucose, 1% xylose, 10 μg/ml tryptophan and lysine. The swimming speed is the average speed of >30 cells.
